Broadly speaking: Cards implemented using web meta tags on your site, will work in the validator and in regular Tweets. Cards on the Ads platform (lead gen, website, or app cards) are managed directly on the ads.twitter.com dashboard and do not require site markup.
If you are asking about something specifically related to the Ads platform, you’ll need to go via the Ads support team (the “Help?” link at the top right of your Ads dashboard should get you support).